Search results for "Exopeptidase activity"
showing 2 items of 2 documents
Exopeptidase digestion in combination with field desorption mass spectrometry for amino acid sequence determination
1982
Numerous studies have been devoted in the last years to the development of mass spectrometric methods for the sequence determination of peptides [ 1,2]. Most advanced among this work has been so far the rigorous chemical derivatization of oligopeptides to achieve sufficient volatility for the application of conventional (electron impact, EI) mass spectrometry [2-41. For example, the analysis by gas chromatography-mass spectrometry (GC-MS) of mixtures of oligopeptide fragments derivatized after chemical or enzymatic hydrolysis of polypeptides has been successfully used for sequence determinations [2,5]. Major limitations of this approach are that only small peptide derivatives are amenable t…
Changes in the Level of Peptidase Activities in Pea Ovaries during Senescence and Fruit Set Induced by Gibberellic Acid
1990
The activities and changes in the levels of exopeptidase and endopeptidase activities were characterized in unpollinated ovaries of Pisum sativum L. cv Alaska during senescence and early fruit development induced by gibberellic acid (GA3). Two aminopeptidases and one iminopeptidase were electrophoretically separated. These peptidases were sensitive to inhibitors of sulfhydryl proteases. Carboxypeptidase activity was inhibited by phenylmethyl sulfonyl fluoride. An azocasein-degrading endopeptidase, sensitive to thiol protease inhibitors, was also found. An increase in the specific activity of aminopeptidase during both fruit development and ovary senescence was observed. In contrast, the spe…